Growth factor-like activity and oncogene regulation of parathyroid hormone-related protein by Xin Li

Cover of: Growth factor-like activity and oncogene regulation of parathyroid hormone-related protein | Xin Li

Published by National Library of Canada in Ottawa .

Written in English

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Edition Notes

Thesis (M.Sc.)--University of Toronto, 1993.

Book details

SeriesCanadian theses = Thèses canadiennes
The Physical Object
Pagination2 microfiches : negative.
ID Numbers
Open LibraryOL15475587M
ISBN 100315924225

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Parathyroid hormone-related protein (PTHrP) is produced by prostate carcinoma cells and tumors, but little is known of its role in prostate carcinogenesis. The goal of this study was to evaluate PTHrP expression in the regulation of prostate carcinoma growth using human and animal models.

PTHrP expression was assessed in prostate cancer cell lines in by: Parathyroid hormone-related protein (PTHrP) was initially identi-fied as the factor responsible for the paraneoplasic syndrome, humoral hypercalcemia of malignancy (4).

PTHrP has been proven to be a polyprotein normally expressed throughout the body where it displays a variety of effects, including the regulation of cellular growth,Cited by: Regulation of insulin-like growth factor-binding protein-4 protease activity by estrogen and parathyroid hormone in SaOS-2 cells: implications for the pathogenesis of postmenopausal osteoporosis.

Kudo Y(1), Iwashita M, Itatsu S, Iguchi T, Takeda by: Parathyroid hormone-related protein (PTHrP), with isoforms ranging from to amino acids, has long been implicated in the development and regulation of multiple tissues, including that of the skeleton, via paracrine and autocrine signaling.

PTHrP is also known as a potent mediator of cancer-induced bone disease, contributing to a vicious cycle between tumor cells and the bone Cited by: 1.

the cellular localization and regulation of this growth factor by the hormone gastrin. Analysis of Pthlh LacZ/+ reporter mice localized Pthlh to parietal cells in the gastric xii.

E. Alsat, J. Haziza, M.L. Scippo, F. Frankenne, D. Evain BrionIncrease in epidermal growth factor receptor and its mRNA levels by parathyroid hormone (1–34) and parathyroid hormone-related protein (1–34) during differentiation of human trophoblast cells in culture. ATLL cells express factors such as interleukin-1, tumor necrosis factor β, parathyroid hormone-related protein (PTHrP), macrophage inflammatory protein-1α (MIP-1α) and receptor activator of nuclear factor-κB ligand (RANKL) that directly and/or indirectly stimulate osteoclast differentiation and activity, resulting in hypercalcemia [].

John Foley, John J Wysolmerski, Caterina Missero, Connie S King, William M Philbrick, Regulation of parathyroid hormone-related protein gene expression in murine keratinocytes by E1A isoforms: a role for basal promoter and Ets-1 site, Molecular and Cellular Endocrinology, /S(99), (), ().

Anthony W. Norman Ph.D., Helen L. Henry Ph.D., in Hormones (Third Edition), C Parathyroid Hormone-Related Protein. Parathyroid hormone-related protein (PTHrP) was originally purified and an N-terminal amino sequence was obtained from human tumors associated with humoral hypercalcemia of malignancy (HHM).

This information permitted its molecular cloning and study of the total amino. Hepatocyte growth factor (HGF) or scatter factor (SF) is a paracrine cellular growth, motility and morphogenic is secreted by mesenchymal cells and targets and acts primarily upon epithelial cells and endothelial cells, but also acts on haemopoietic progenitor cells and T has been shown to have a major role in embryonic organ development, specifically in myogenesis, in adult.

Parathyroid hormone-related protein (PTHrP) is produced by a wide range of neoplastic and normal cells, including keratinocytes where it may be involved in the regulation of cellular growth and.

Hypercalcemia, one of the most common paraneoplastic syndromes, is generally caused by tumor secretion of parathyroid hormone-related protein (PTHrP). PTHrP isoforms exhibit a high degree of homology within the first 13 residues to the amino (N)-terminus of parathyroid hormone (PTH).

Clear cell renal carcinoma (CCRC) is responsible for 2% of cancer-related deaths worldwide and is resistant to virtually all therapies, indicating the importance of a search for new therapeutic targets. Parathyroid hormone-related protein (PTHrP) is a polyprotein derived from normal and malignant cells that regulates cell growth.

In the current study, we show that blocking PTHrP with. The modeling of long bone surfaces during linear growth is a key developmental process, but its regulation is poorly understood. We report here that parathyroid hormone-related peptide (PTHrP.

Abstract. Parathyroid hormone-related protein (PTHrP) is widely expressed in fetal and adult tissues and is a key regulator for cellular calcium transport and smooth muscle cell contractility, as well as a crucial control factor in cell proliferation, development and differentiation.

Regulation of oncogene expression in cultured aortic smooth muscle cells. Post-transcriptional control of c-myc mRNA. J Biol Chem. Sep 25; (27)– Ikeda K, Lu C, Weir EC, Mangin M, Broadus AE. Transcriptional regulation of the parathyroid hormone-related peptide gene by glucocorticoids and vitamin D in a human C-cell line.

Co-expression of parathyroid hormone related protein and TGF-beta in breast cancer predicts poor survival outcome. TGF-β has been recognized to be a multi-functional growth factor involved in regulation of such processes as development, wound healing, we consider both TGF-β and PTHrP as oncogenes in breast cancer.

Allele-specific patterns of the mouse parathyroid hormone-related protein: Influences on cell adhesion and migration Article (PDF Available) in Oncogene 22(49) November with 51 Reads. Abstract. Ina second member of the parathyroid hormone (PTH) family, PTH-related protein (PTHrP), was identified and cloned (1).PTHrP is produced in many normal fetal (2–11) and adult (7,12–19) tissues, including several endocrine tissues, cartilage and bone, heart, vascular and other smooth muscle, skin, central nervous system, liver, kidney, and lung.

Effects of parathyroid hormone and parathyroid hormone–related protein on bone cells Survival. Cell culture studies show that PTH rapidly stimulates transcription of the prosurvival gene Bcl-2 while increasing the inactivation of the apoptotic protein Bad (Bellido et al.,Jilka et al., ), suggesting that activation of the PTHR in osteoblasts may inhibit apoptosis.

Indeed, the complex growth factor-like properties of PTHrP has shed new light onto potential roles of this peptide in the regulation of tumor growth and invasion.

Initial studies in breast, prostate and lung cancer and recent results in renal cell carcinoma (RCC) suggest such roles and highlight the therapeutic potential of PTHrP-targeting strategies in human cancer including RCC.

Parathyroid hormone-related protein modulates the effect of transforming growth factor-beta on deoxyribonucleic acid and collagen synthesis in fetal rat bone cells. Centrella M(1), Canalis E, McCarthy TL, Stewart AF, Orloff JJ, Insogna KL.

The parathyroid hormone-related protein (PTHrP) gene (Pthlh) maps in the distal region of mouse chromosome 6 that contains a quantitative trait locus associated with genetic predisposition to skin. Parathyroid hormone-related protein (or PTHrP) is a protein member of the parathyroid hormone family secreted by mesenchymal stem is occasionally secreted by cancer cells (breast cancer, certain types of lung cancer including squamous-cell lung carcinoma).However, it also has normal functions in bone, tooth, vascular and other tissues.

Parathyroid hormone-related protein (PTHrP) is a growth factor that was first isolated in from tumors of patients with humoral hypercalcemia [7– 9]. PTHrP and parathyroid hormone (PTH) share structural homology at their respective amino termini, enabling PTHrP to interact with classical PTH receptors [ 10, 11 ].

Abstract. PTH induces the synthesis of insulin-like growth factor I (IGF-I) and regulates the expression of IGF-binding proteins (IGFBP) in osteoblast cultures.

Parathyroid hormone-related peptide (PTHrP) was originally discovered as a systemic humoral factor released by tumor cells that were responsible for humoral hypercalcemia of malignancy.

Parathyroid. In osteoblastic cells, transforming growth factor beta1 (TGF-beta1) has been found to regulate the expression of a variety of proto-oncogenes including c-fos, c-jun, and junB. The c-fos in particular has been implicated in the mitogenic effect of TGF-beta1. Here, we examined the role of these early.

Section I Parathyroid Gland, Ultimobranchial Gland, and Stannius Corpuscle Hormones Chapter Parathyroid Hormone Family 35A. Parathyroid Hormone 35B. Parathyroid Hormone-Related Protein 35C.

Tuftelin-interacting protein 39 Chapter Calcitonin/Calcitonin Gene-Related Peptide Family 36A. Calcitonin 36B. Calcitonin Gene-Related Peptide 36C. Parathyroid hormone (PTH) and parathyroid-hormone-related protein (PTHrP) are two predominant members of the PTH family of peptides with major roles in calcium homeostasis and bone formation.

PTH was discovered in the acid extracts of parathyroid glands because it restored the blood calcium level in the parathyroidectomized dog [24]. ATLL cells express factors such as interleukin-1, tumor necrosis factor β, parathyroid hormone-related protein (PTHrP), macrophage inflammatory protein-1α (MIP-1α) and receptor activator of nuclear factor-κB ligand (RANKL) that directly and/or indirectly stimulate osteoclast differentiation and activity, resulting in hypercalcemia [20–24].

PTHrP (parathyroid hormone-related protein) overexpression by prostate carcinoma cells has been implicated in tumor progression. Although the biological effects of PTHrP can be mediated by the G-protein-coupled PTH/PTHrP receptor, PTHrP also has intracrine actions mediated by a nuclear localization sequence at residues 87– We investigated the effect of PTHrP transfection and.

Parathyroid hormone-related protein (PTHrP), a polyprotein discovered inis the major causative agent in humoral hypercalcemia of malignancy associated to a broad range of tumors (3). However, the complex growth factor-like properties of PTHrP have shed new light onto potential roles of this peptide in the regulation of tumor growth and.

Parathyroid hormone-related protein (PTHrP), classically regarded as the mediator of the humoral hypercalcemia of malignancy syndrome, is a polyhormone that undergoes proteolytic processing into smaller bioactive forms. These bioactive forms comprise an N-terminal- as well as midregion- and C-terminal peptides, which have been shown to regulate various biological events, such as survival.

Abbas Abdollahi, Rudi Bao, Thomas C Hamilton, LOT1 is a growth suppressor gene down-regulated by the epidermal growth factor receptor ligands and encodes a nuclear zinc-finger protein, Oncogene, /, 18, 47, (), ().

Abstract. We have shown that parathyroid hormone-related protein (PTHrP) is a survival factor for human renal cell carcinoma (RCC) and that its expression is negatively regulated by the von Hippel-Lindau (VHL) tumor suppressor gene at the level of messenger RNA (mRNA) stability, as observed for tumor growth factors (TGFs).

Parathyroid hormone-related protein (PTHrP) is a causative factor of humoral hypercalcemia in breast cancer and other malignancies.

We studied circulating PTHrP levels with three different immunoassays di rected against different parts of the PTHrP molecule in 48 patients with breast cancer and eucalcemia.

The methods used were: (a) a RIA with. Parathyroid hormone-related protein (PTHrP) is expressed in a variety of human cancers including lung cancer.

Three mature peptides with different COOH-terminal regions, PTHrP (1–), PTHrP (1–), and PTHrP (1–), are translated from three different mRNAs through alternative splicing. In each, COOH-terminal fragment (C-PTHrP) is stable and measurable in the urine.

The paraneoplastic endocrine syndromes (“ectopic” or “inappropriate” hormone production) comprise a wide array of symptom complexes associated with malignant or less commonly benign neoplasms. Most of the syndromes are associated with the production of peptide hormones, which, in some instances, have autocrine stimulatory effects.

Hypercalcemia, the most common paraneoplastic endocrine. On the basis of studies of the biologic action of PTHRP in cultured human keratinocytes and in SKH-1 hairless mice, Holick et al. () produced strong evidence that PTHRP is an endogenous antiproliferative factor that participates in the regulation of epidermal and hair follicle cell growth.

They suggested that the antiproliferative activity of PTH-(), a PTH fragment, and PTHRP-(. Parathyroid hormone‐related peptide (PTHrP) suppresses the maturation. Bone morphogenetic protein‐2 (BMP‐2), ‐4, or ‐7 induces maturation of proliferating chondrocytes, and this promoting effect is dominant over the suppressive effect of PTHrP.

These BMP induce the expression of both the Ihh and Noggin genes. As Noggin protein.A RIA for PTH-related protein (PTHrP) is described, using a polyclonal goat antiserum against synthetic PTHrP-() and recombinant PTHrP-() as standard.

The detection limit is 2 pmol/L, and intra- and interassay coefficients of variation are % and %, respectively.parathyroid hormone 1 receptor (PTH1R) is the classical PTH receptor, is expressed in high levels in bone and kidney and regulates calcium ion homeostasis through activation of adenylate cyclase and phospholipase C.

parathyroid hormone 2 receptor (PTH2R) is expressed primarily in the central nervous system, pancreas, testis, and placenta.

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